Running a Fisher's F-test in XLSTAT to assess the equality of variance of 2 samples

An Excel sheet with both the data and the results can be downloaded by clicking here.

The data are from [Fisher M. (1936). The Use of Multiple Measurements in Taxonomic Problems. Annals of Eugenics, 7, 179 -188] and correspond to the sepal characteristics of 100 Iris flowers described by two variables (sepal length, sepal width). There are two different species included in this example: setosa and versicolor.

Our goal is to assess if there is a difference between the species for the sepal length and sepal width. We will then compare the distribution of these variables for the 2 samples.

The first thing to do is to assess if the samples follow a Normal distribution as the Fisher F-test is sensitive to data that do not follow a normal distribution.

You will find those statistics computed in the Excel sheet. All 4 samples (Versicolor-Sepal length, Versicolor-Sepal width, Setosa-Sepal length, Setosa-Sepal width) follow a normal distribution.

Then we do a F-test to know if the variance are equal. If the variances are equal we can do a test to compare the averages.

To realize a two-sample comparison of variances test go to the menu bar Parametric Tests / Two-sample comparison of variances.


In the Two-sample comparison of variances dialog box, in the tab General select the data for the sample 1 and 2. For Sample 1 select the column B containing the sepal length for the variety Versicolor and for theSample 2 the column E corresponding to the sepal length for the Setosa samples.

The Data format is One column per sample as each column corresponds to one of the samples.

We select the option Sheet to get the results in a new sheet of the workbook.

As the columns have a label the option Column labels should be enabled.

The test we decide to run is the Fisher’s F-test.

F-test using XLSTAT - Data Analysis Software

Once all these options are set we can move on to the tab Options.

We want to test the equality of variance which means that we need to test the alternative hypothesis:Variance 1 / Variance 2 ≠ R where R is 1.

The default significance level of 5% is to be kept.

F-test using XLSTAT - Statistical Analysis Package

We don’t have missing data so we can go directly to the tab Outputs and enable the only available option:Descriptive statistics.

F-test using XLSTAT - Statistical Analysis Software

Press OK, when everything is set.

Results of a Fisher's F-test in XLSTAT to assess the equality of variance of 2 samples

The results that appear in a new sheet show that the H0 hypothesis should be rejected as the p-value 0.009 is inferior to our limit of 5%. Hence the variances cannot be considered as equal. The two populations -Versicolor and Setosa - sepal length do not follow the same distribution.

We are now going to do the same thing but for the sepal width.

The only change in the procedure described above is the data selection.
For Sample 1 enlighten the column C and for Sample 2 choose the column F.

F-test using XLSTAT - Statistics Software

This time the variances can be considered as equal as the p-value of the test (0.189) is superior to 0.05.

F-test using XLSTAT - Statistics Excel

As the equality of variance or homoscedasticity is assumed we can run a test of comparison of mean.

You can notice in the Descriptive statistic table that the mean of the sepal width for Versicolor is inferior to the mean of Setosa for the same characteristic. Therefore we can run a one-tailed test for the test on the average.

Go to the menu Parametric tests / Two-sample t-test and z-test

F-test using XLSTAT - Stats Excel

In the general tab do the same sample selection as previously for the sepal width.

Select the option Student’s test as we do not know the true variances of the populations.

F-test using XLSTAT - Statistics Excel

In the Options tab elect the alternative Mean 1 – Mean 2 < D where D is 0.

We can Assume equality for the variances as we just computed the test before.

F-test using XLSTAT - Statistics Excel

Click on OK.

As can be seen in the results of this test, we conclude that there is significant difference between the two means, the sepal width of Versicolor iris being smaller than the sepal width of the Setosa iris. The two populations -Versicolor and Setosa - sepal width do not follow the same distribution.

F-test using XLSTAT - Statistics Excel

Click here for other tutorials.

About KCS

Kovach Computing Services (KCS) was founded in 1993 by Dr. Warren Kovach. The company specializes in the development and marketing of inexpensive and easy-to-use statistical software for scientists, as well as in data analysis consulting.

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